Progress Report · Summer 2025 – Fall 2025

Hamlin, TX Grapefruit &
TX Juvenile Valencia Results

University of Florida Institute of Food and Agricultural Sciences · UF/IFAS Citrus REC, Lake Alfred, FL Davie M. Kadyampakeni, Associate Professor · Spring 2026 Report

Executive Summary

Hamlin & Grapefruit Trials — Expanded Multi-State Results

This second progress report covers new data collected during Summer and Fall 2025 across Florida Hamlin groves and Texas grapefruit and juvenile Valencia sites. It expands upon the Spring 2025 Valencia results, bringing three varieties and multiple seasons of evidence into the CitruSaver picture.

Hamlin fruit yield generally increased with biostimulant-based treatments compared with control and BM alone. CS1 consistently trended toward higher yields. CS2 + BM showed the highest or among the highest yields in several cases, suggesting a positive additive effect of combining biostimulants with beneficial microbes.

Canopy volume increased from spring to fall across all locations. Fruit quality parameters — soluble solids, titratable acidity, ripening index, and juice content — were highly stable across treatments at all sites.

Overall Executive Takeaway

Biostimulant treatments — especially CS1 and CS2 combined with beneficial microbes — support improved tree performance primarily through increased yield and canopy development, with minimal effects on fruit quality. Seasonal and site-specific management strongly influenced outcomes, indicating these treatments are most effective when integrated into location-specific strategies rather than applied as a one-size-fits-all solution.

Florida vs. Texas — Inverse Relationship Observed

Florida

CS1 > CS2

CS1 outperforms CS2 at Hamlin Wauchula and Fort Meade sites

Texas

CS2 > CS1

CS2 outperforms CS1 in Texas grapefruit trials — site-dependent response

This Report Covers

  • FL Hamlin — Wauchula & Fort Meade yield, canopy, fruit drop
  • TX Grapefruit — mature tree yield & canopy volume
  • TX Juvenile Valencia — early-stage canopy development
  • Fruit quality across all sites (Brix, TA, RI, juice per box)
  • Fruit drop tracking — 3 dates, 2 Florida Hamlin sites
Hamlin Yield — Florida

CS1 Led Yield at Wauchula · BM & CS1 Led at Fort Meade

Fruit yield in kg per tree (Figure 1). Treatment effects on Hamlin yield are site-dependent — CS1 was the top performer at Wauchula, while BM and CS1 shared the lead at Fort Meade under less favorable growing conditions.

Wauchula — Hamlin Oranges

Summer–Fall 2025

+30.2%

CS1 vs Control

CS1 (best) CS2+BM Control
CS1 (CitruSaver 1) 32.8 kg/tree · +30.2%
32.8 kg
CS2 + BM 30.1 kg/tree
30.1 kg
CS2 26.5 kg/tree
26.5 kg
BM (Microbes) 24.5 kg/tree
24.5 kg
Control (untreated) 25.2 kg/tree · baseline
25.2 kg

Key takeaway: Soil biostimulants, especially CS1, are associated with increased fruit yield. Adding beneficial microbes to CS2 further improves performance. CS2+BM was the second highest result.

Fort Meade — Hamlin Oranges

Summer–Fall 2025

+17.1%

BM vs Control

BM (best here) CS1 Control
BM (Microbes only) 13.7 kg/tree · +17.1%
13.7 kg
CS1 (CitruSaver 1) 12.3 kg/tree · +5.1%
12.3 kg
Control 11.7 kg/tree · baseline
11.7 kg
CS2 8.2 kg/tree
8.2 kg
CS2 + BM 8.0 kg/tree
8.0 kg

Note: Fort Meade shows lower overall yields than Wauchula — less favorable growing conditions. CS2-based treatments were less effective here, reflecting site- and management-dependent variability. CS1 still outperformed control.

Texas Grapefruit Yield

Progressive Yield Gains — CS2+BM Best at 19.1%

Texas grapefruit yield increased progressively from BM and Control to the biostimulant treatments. CS2 and CS2+BM outperformed CS1 in Texas — the inverse of what was observed in Florida — indicating a region-specific treatment response likely tied to soil conditions, climate, and tree history.

CS2 + BM (best) 174.5 kg/tree · +19.1%
174.5 kg · +19.1%
CS2 (CitruSaver 2) 171.9 kg/tree · +17.3%
171.9 kg · +17.3%
CS1 (CitruSaver 1) 160.8 kg/tree
160.8 kg
Control 146.5 kg/tree · baseline
146.5 kg
BM (Microbes) 142.7 kg/tree
142.7 kg

TX Grapefruit Key Finding

+19.1%

CS2 + BM vs. untreated control

The combined application of CitruSaver 2 with beneficial microbes produced the highest yield — indicating an additive or synergistic effect when biostimulants and microbes are applied together.

Florida–Texas Comparison

Treatment Florida (CS1 leads) Texas (CS2 leads)
CS1 ✓ Top performer Mid-range
CS2 Weaker in FL ✓ +17.3%
CS2+BM 2nd at Wauchula ✓ +19.1% Best

Results are site-dependent and influenced by soil conditions, tree health, and management. Further research will clarify region-specific optimal formulations.

Fruit Quality

Yield Gains Without Quality Loss — Across All Three Varieties

Fruit quality parameters — soluble solids (°Brix), titratable acidity, TSS/TA ripening index, and juice per box — were statistically identical across all treatments at all three sites. This consistent finding held across Wauchula Hamlins, Fort Meade Hamlins, and Texas Grapefruit.

Wauchula Hamlins

Florida · Fall 2025

Soluble Solids (°Brix) ~8.7° · No diff.
Titratable Acidity ~0.57% · Stable
TSS/TA Ratio (RI) ~15.5 · No diff.
Juice Per Box (lbs) ~46 lbs · Stable

Fort Meade Hamlins

Florida · Fall 2025

Soluble Solids (°Brix) ~9.2° · No diff.
Titratable Acidity ~0.66% · Stable
TSS/TA Ratio (RI) ~2.3 · No diff.
Juice Per Box (lbs) ~46 lbs · Stable

Texas Grapefruit

Mature trees · Fall 2025

Soluble Solids (°Brix) ~9.4° · No diff.
Titratable Acidity ~0.86% · Stable
TSS/TA Ratio (RI) ~11.0 · No diff.
Juice Per Box (lbs) ~41 lbs · Stable

Consistent finding across all sites and varieties

Fruit quality parameters were highly stable across treatments for Wauchula Hamlins, Fort Meade Hamlins, and Texas Grapefruit. No consistent upward or downward trends were observed. This confirms that yield and canopy improvements occurred without any compromise in fruit quality — a critical finding for commercial grower adoption.

Canopy Volume — Spring & Fall 2025

CS1 Consistently Grew the Largest Canopies

Canopy volume (m³) was measured in both Spring and Fall 2025 across all sites. All sites showed strong seasonal canopy expansion. CS1 consistently trended toward the largest canopy volumes — especially at the Wauchula Hamlin site.

Wauchula Hamlin

Spring → Fall 2025

CS1 Best

Both seasons

Spring 2025

CS1
28.3 m³
CS2+BM
23.9 m³
BM
24.6 m³
Control
23.4 m³
CS2
17.7 m³

Fall 2025

CS1
26.6 m³
CS2+BM
23.7 m³
BM
24.0 m³
Control
22.6 m³
CS2
17.5 m³

Fort Meade Hamlin

Spring → Fall 2025

Statistically similar

No significant differences among treatments at Fort Meade in either season. BM and CS1 showed numerically larger canopy volumes, but all treatments remained statistically similar — indicating stable canopy development across the board regardless of treatment.

Spring 2025 BM (highest)

37.0 m³

Fall 2025 BM (highest)

34.1 m³

Texas — Grapefruit & Juvenile Valencia

Fall 2025 Canopy Expansion

TX Grapefruit (mature) — Fall 2025

BM

47.9

Control

46.1

CS1 +8%

48.8

CS2 +9%

48.6

CS2+BM

48.1

TX Juvenile Valencia — Fall 2025 (m³)

BM

1.4

Control

1.5

CS1 +6.7%

1.6

CS1+BM

1.3

Juvenile Valencia: minimal differences at this early stage. CS1 shows slight advantage by Fall 2025.

Fruit Drop Monitoring

CS2 & CS2+BM Trended Toward Lower Late-Season Fruit Drop

Fruit drop counts were measured at three sampling dates — October 16, October 29, and November 25, 2025 — at both Fort Meade and Wauchula Hamlin sites. Patterns were consistent across sites.

CS2 & CS2+BM — Lower Drop (Late Season)

Both Fort Meade and Wauchula sites: CS2 and CS2+BM trended toward lower fruit drop at later sampling dates (Oct 29, Nov 25). Differences were not statistically significant but were consistent.

CS1 — Slightly Higher Early-Season Drop

CS1 occasionally showed numerically higher early-season fruit drop. These differences diminished later in the season — by the final sampling date (Nov 25), all treatments converged to similar low counts.

No Statistically Significant Differences

Mean separation (Tukey HSD, p ≤ 0.05) showed no significant differences in fruit drop across treatments at either site. Observed trends are directional, not conclusive.

Sampling Schedule — Hamlin Fruit Drop

October 16, 2025 — First Count

Fort Meade & Wauchula · Moderate fruit drop observed at both sites · CS1 slightly higher at Wauchula early counts

October 29, 2025 — Second Count

Fort Meade: CS2 & CS2+BM showed lower counts · Wauchula: similar pattern developing · differences minor

November 25, 2025 — Final Count

Both sites: fruit drop generally lower by late season · All treatments converged · CS2 & CS2+BM maintained lower trend

Treatment Fruit Drop Tendency

CS2 / CS2+BM ↓ Lower late-season
BM / Control → Intermediate
CS1 ↑ Slightly higher early (then converges)

Source: UF/IFAS Progress Report — "Assessing the therapeutic effects of CitruSaver on the productivity and health of HLB-affected citrus trees," Davie M. Kadyampakeni, Associate Professor, UF/IFAS Citrus REC, Lake Alfred, FL. Progress Report: FL Hamlin, TX Grapefruit, TX Juvenile Valencia, Summer 2025–Fall 2025 (Spring 2026 publication). Results are preliminary. Treatment effects are site- and management-dependent and may not reflect all growing conditions.